Structure and Function of Human Erythrocyte Pyruvate Kinase
نویسندگان
چکیده
منابع مشابه
Structure and function of human erythrocyte pyruvate kinase. Molecular basis of nonspherocytic hemolytic anemia.
Deficiency of human erythrocyte isozyme (RPK) is, together with glucose-6-phosphate dehydrogenase deficiency, the most common cause of the nonspherocytic hemolytic anemia. To provide a molecular framework to the disease, we have solved the 2.7 A resolution crystal structure of human RPK in complex with fructose 1,6-bisphosphate, the allosteric activator, and phosphoglycolate, a substrate analog...
متن کاملPurification of human erythrocyte pyruvate kinase.
We describe a purification scheme for human erythrocyte pyruvate kinase. Following two ammonium sulfate fractionations, batchwise absorption on CM-cellulose, chromatography on CM-cellulose with fructose 1,6-diphosphate elution, and chromatography on DEAE-cellulose, a 30,000fold purification was achieved with a yield of 8% from the original hemolysate. Antiserum against partially purified pyruva...
متن کاملThe structure and function of pyruvate kinase.
Hoggett, G. L. & Kellett, J. E. (1976) Bur. J. Biochem. 66,65-77 Lazarus, N. K., Derechin, M. & Barnard, E. A. (1968) Biochemistry 7, 2390-2400 McDonald, R. C., Steitz, T. A. & Engleman, D. M. (1979) Biochemistry 18,338-342 Menezes, L. C. & Pudles, J. (1977) Arch. Biochem. Biophys. 178, 34-42 Morris, H. R. & Dell, A. (1975) in Instrumentation in Amino Acid Sequence Analysis (Perham, R. N., ed.)...
متن کاملIdentification of novel allosteric regulators of human-erythrocyte pyruvate kinase.
Erythrocyte pyruvate kinase (PK) is an important glycolytic enzyme, and manipulation of its regulatory behavior by allosteric modifiers is of interest for medicinal purposes. Human-erythrocyte PK was expressed in Rosetta cells and purified on an Ni-NTA column. A search of the small-molecules database of the National Cancer Institute (NCI), using the UNITY software, led to the identification of ...
متن کاملRapid determination of erythrocyte pyruvate kinase activity.
We report a new potentiometric method for determining pyruvate kinase (PK). Enzymatic activity is measured by monitoring the change in pH produced in the reaction buffer under International Committee for Standardization in Haematology (ICSH) standardized assay conditions, and the lactate dehydrogenase reaction is automatically subtracted in each measuring cycle. The analysis, performed at 37 de...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 2002
ISSN: 0021-9258
DOI: 10.1074/jbc.m202107200